Novel dual-emissive fluorescent immunoassay for synchronous monitoring of okadaic acid and saxitoxin in shellfish

Mingming Yin; Weijie Wang; Jie Wei; Xiaomei Chen; Quansheng Chen; Xi Chen; Munetaka Oyama

doi:10.1016/j.foodchem.2021.130856

Novel dual-emissive fluorescent immunoassay for synchronous monitoring of okadaic acid and saxitoxin in shellfish

Food Chem. 2022 Jan 30:368:130856. doi: 10.1016/j.foodchem.2021.130856. Epub 2021 Aug 13.

Authors

Mingming Yin¹, Weijie Wang¹, Jie Wei², Xiaomei Chen³, Quansheng Chen⁴, Xi Chen⁵, Munetaka Oyama⁶

Affiliations

¹ College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
² College of Food and Biological Engineering, Jimei University, Xiamen 361021, China. Electronic address: xmchen@jmu.edu.cn.
³ College of Food and Biological Engineering, Jimei University, Xiamen 361021, China. Electronic address: jiewei@jmu.edu.cn.
⁴ College of Food and Biological Engineering, Jimei University, Xiamen 361021, China; School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: qschen@ujs.edu.cn.
⁵ State Key Laboratory of Marine Environmental Science, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
⁶ Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8520, Japan.

PMID: 34425333
DOI: 10.1016/j.foodchem.2021.130856

Abstract

In this work, we present a novel dual-emissive fluoroimmunoassay for synchronous monitoring of okadaic acid (OA) and saxitoxin (STX) using multicolor fluorescent labels composed of sulfur, phosphorous co-doped graphene quantum dots (S, P-GQDs), and ovalbumin (OVA)-coated gold nanoparticles (OVA-AuNPs). The novel OVA-AuNPs were prepared by the reduction of chloroauric acid under alkaline conditions using OVA as a reducing agent. Both S, P-GQDs and OVA-AuNPs exhibit bright fluorescence, more importantly, a large emission wavelength difference (Δλ = 156 nm) under an excitation of 400 nm and relatively independent fluorescence behavior, which are essential to realizing the dual-signal marks in a directly mixing system. Using a competitive fluorescence-linked immunosorbent assay (cFLISA) format, the dual-emissive cFLISA was successfully utilized to measure OA and STX contents in Alectryonella plicatula (commonly named as fingerprint oyster) and the detection results were in good agreement with the commercial enzyme-linked immunosorbent assay (ELISA) kits.

Keywords: Competitive fluorescent immunosorbent assay; Doped graphene quantum dots; Gold nanoparticles; Okadaic acid; Saxitoxin.

MeSH terms

Gold
Graphite*
Immunoassay
Metal Nanoparticles*
Okadaic Acid
Quantum Dots*
Saxitoxin
Shellfish / analysis

Substances

Okadaic Acid
Saxitoxin
Gold
Graphite