Extraction optimization and structural characterization of pectin from persimmon fruit (Diospyros kaki Thunb. var. Rojo brillante)

Carbohydr Polym. 2021 Nov 15:272:118411. doi: 10.1016/j.carbpol.2021.118411. Epub 2021 Jul 8.

Abstract

In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time. The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples. All persimmon pectins showed a molecular weight and galacturonic acid content upper than 328 kDa and 78%, respectively, indicating their suitability as food ingredient. Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples. Remarkably, both pulp tissues (AS and NAS) presented the highest levels of glucose and mannose, non-pectic carbohydrates. In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.

Keywords: Astringent fruits; FTIR spectroscopy; Monosaccharide composition; Optimization; Persimmon; Yield.

MeSH terms

  • Arabinose / analysis
  • Diospyros / chemistry*
  • Fruit / chemistry*
  • Glucose / analysis
  • Hexuronic Acids / analysis
  • Mannose / analysis
  • Molecular Weight
  • Particle Size
  • Pectins / analysis*
  • Rhamnose / analysis
  • Rheology / methods
  • Sodium Citrate / chemistry
  • Spectroscopy, Fourier Transform Infrared / methods
  • Viscosity

Substances

  • Hexuronic Acids
  • Sodium Citrate
  • galacturonic acid
  • Pectins
  • Arabinose
  • Glucose
  • Mannose
  • Rhamnose