Application of spectroscopic techniques and chemometric methods to differentiate between true cinnamon and false cinnamon

Abstract

The objective of this study was to identify useful chemical markers for the differentiation of true and false cinnamon; to develop a fast and efficient method of differentiation using MIR associated with chemometric methods. A total of 129 cinnamon samples from 3 countries (Brazil, Sri Lanka, and Paraguay) were purchased, being differentiated by visual analysis. The bioactive compounds, antioxidant activity, HPLC, and MIR analysis were performed. The data obtained were submitted to PCA and PLS-DA. The results showed that the samples of true cinnamon have a higher concentration of eugenol, cinnamaldehyde, and antioxidant capacity, as well as a lower coumarin content. The PCA showed the separation of two groups of cinnamon samples. PLS-DA was effective in differentiating the studied samples with an accuracy of 94.44% and 100% for the composition and MIR analysis, respectively. The MIR proved to be an alternative for the rapid differentiation of true and false cinnamon.

Keywords: Adulteration; Chemometric; Coumarin; Eugenol; MIR.