Potential of Autologous Progenitor Cells and Decellularized Porcine Artery Matrix in Construction of Tissue-engineered Vascular Grafts

Jieh-Neng Wang; Chung-Dann Kan; Shao-Hsien Lin; Ko-Chi Chang; Stephanie Tsao; Tak-Wah Wong

doi:10.1080/15476278.2021.1963603

Potential of Autologous Progenitor Cells and Decellularized Porcine Artery Matrix in Construction of Tissue-engineered Vascular Grafts

Organogenesis. 2021 Oct 2;17(3-4):72-84. doi: 10.1080/15476278.2021.1963603. Epub 2021 Aug 18.

Authors

Jieh-Neng Wang¹, Chung-Dann Kan², Shao-Hsien Lin¹, Ko-Chi Chang¹, Stephanie Tsao³, Tak-Wah Wong^{3

4

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Affiliations

¹ Departments Of Pediatrics, National Cheng Kung University Hospital, College Of Medicine, National Cheng Kung University, Tainan, Taiwan.
² Departments Of Surgery, National Cheng Kung University Hospital, College Of Medicine, National Cheng Kung University, Tainan, Taiwan.
³ Department Of Dermatology, National Cheng Kung University Hospital, College Of Medicine, National Cheng Kung University, Tainan, Taiwan.
⁴ Department Of Biochemistry And Molecular Biology, College Of Medicine, National Cheng Kung University, Tainan, Taiwan.
⁵ Center Of Applied Nanomedicine, National Cheng Kung University, Tainan, Taiwan.

Abstract

To develop a tissue-engineered vascular graft, we used pericardial effusion-derived progenitor cells (PEPCs) collected from drained fluid after open-heart surgery in children with congenital heart diseases to repopulate a decellularized porcine pulmonary artery. The PEPCs were compared with human fibroblasts (HS68) and human umbilical vein endothelial cells (HUVECs) in cell growth and migration. They were cultured with the matrices via an inner approach (intima), lateral approach (media), and outer approach (adventitia). PEPCs grew and migrated better than the other two cells 14 days after seeding in the decellularized vessel. In immunofluorescence assays, PEPCs expressed CD90 and CD105 indicating a vascular differentiation. PEPCs grew in a decellularized porcine pulmonary artery matrix may have the potential for producing tissue-engineered vascular grafts.

Keywords: Tissue-engineered vascular graft; decellularized biological scaffolds; pericardial effuse drain fluid; progenitor cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arteries
Blood Vessel Prosthesis*
Human Umbilical Vein Endothelial Cells
Humans
Stem Cells
Swine
Tissue Engineering*
Tissue Scaffolds

Grants and funding

This work was supported by the Ministry of Science and Technology, Taiwan [MOST 108-2314-B-006 -065 -MY2]; Ministry of Science and Technology, Taiwan [MOST-109-2327-B-006-005]; Ministry of Science and Technology, Taiwan [MOST-110-2314-B-006-048-MY2]; Ministry of Science and Technology, Taiwan [MOST-103-2314-B-006-077]; National Cheng Kung University Hospital [1106018]; National Cheng Kung University Hospital [10506007].