Snuella sedimenti sp. nov., isolated from marine sediment

Abstract

A Gram stain-negative, aerobic, motile by gliding, rod-shaped bacterial strain CAU 1569^T was isolated from marine sediment on Shido Island in Incheon. It grew at 20-37 °C (optimum, 30 °C), pH 6.0-9.0 (optimum, 7.0), 2-6% NaCl (w/v) (optimum, 2%). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CAU 1569^T formed a distinct lineage with only the type strain of Snuella. Strain CAU 1569^T showed high similarity to S. lapsa KACC 14152^T (95.8%), Mariniflexile gromovii KMM KCTC 12570^T, Aestuariibaculum marinum KCTC 52521^T (95.4%), A. suncheonense KACC 16186^T (94.6%) and Yeosuana aromativorans KCCM 42019^T (94.4%). The genome contained 57 contigs, 3,437 protein-coding gene, 3 rRNAs (5, 16, and 23S), 43 tRNAs, and with a 35.7 mol% G + C content. The DDH value between strain CAU 1569^T and S. lapsa KACC 14152^T was 39.4 ± 0.6%. The only isoprenoid quinone was menaquinone 6 (MK-6). The major fatty acids were iso-C_15:0, C_15:1-iso G, and C_17:0 iso 3-OH. Strain CAU 1569^T contained diphosphatidylglycerol, aminoglycolipid, unidentified aminolipid, and three unidentified lipids. Based on phylogenetic, genomic, physiologic, and chemotaxonomic characterizations, strain CAU 1569^T represents a novel Snuella species, which the name Snuella sedimenti sp. nov. is proposed. The type of strain is CAU 1569^T (= KCTC 82409^T = MCCC 1K05670^T).

Keywords: Flavobacteriaceae; Marine sediment; Snuella sedimenti sp. nov..