Fluorescent in situ mRNA detection in the adult mouse cochlea

STAR Protoc. 2021 Aug 7;2(3):100711. doi: 10.1016/j.xpro.2021.100711. eCollection 2021 Sep 17.

Abstract

Ossification and the delicateness of the cochlear duct make histologic assessments of the mature cochlea a challenging endeavor. Treatments to soften the bone facilitate sectioning and dissection of the cochlear duct but limit in situ mRNA detection in such specimens. Here, we provide a protocol for in situ mRNA detection using hybridization chain reaction in whole-mount preparations of the adult mouse cochlea. We show examples for multi-probe detection of different mRNAs and describe combination of this method with conventional immunohistochemistry.

Keywords: Antibody; In Situ Hybridization; Microscopy; Molecular Biology; Neuroscience.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cochlea / diagnostic imaging*
  • Cochlea / metabolism
  • Immunohistochemistry / methods*
  • In Situ Hybridization / methods
  • In Situ Hybridization, Fluorescence / methods*
  • Mice
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics

Substances

  • RNA, Messenger