Lipid composition and molecular species of phospholipid in oyster Crassostrea lugubris (Sowerby, 1871) from Lang Co Beach, Hue Province, Vietnam

Thanh Tra Thi Le; Quoc Toan Tran; PeteVladimirovich Velansky; Tien Duc Dam; Long Giang Bach; Long Quoc Pham

doi:10.1002/fsn3.2385

Lipid composition and molecular species of phospholipid in oyster Crassostrea lugubris (Sowerby, 1871) from Lang Co Beach, Hue Province, Vietnam

Food Sci Nutr. 2021 Jun 14;9(8):4199-4210. doi: 10.1002/fsn3.2385. eCollection 2021 Aug.

Authors

Thanh Tra Thi Le^{1

2

3}, Quoc Toan Tran^{1

2}, PeteVladimirovich Velansky⁴, Tien Duc Dam⁵, Long Giang Bach^{6

7}, Long Quoc Pham^{1

2}

Affiliations

¹ Institute of Natural Products Chemistry Vietnam Academy of Science and Technology Hanoi City Vietnam.
² Graduate University of Science and Technology Vietnam Academy of Science and Technology Hanoi City Vietnam.
³ Department of Chemical Engineering Faculty of Chemistry and Environment Thuyloi University Hanoi City Vietnam.
⁴ A.V. Zhirmunsky Institute of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences Vladivostok Russia.
⁵ Institute of Marine Environment and Resources Vietnam Academy of Science and Technology Hanoi city Vietnam.
⁶ NTT Hi-Tech Institute Nguyen Tat Thanh University Ho Chi Minh City Vietnam.
⁷ Center of Excellence for Biochemistry and Natural Products Nguyen Tat Thanh University Ho Chi Minh City Vietnam.

Abstract

Oysters are widely distributed worldwide, but are mainly concentrated in tropics and subtropics. Total lipid (TL), fatty acid (FA) composition of TL and polar lipid (PoL) fractions, phospholipid (PL) class, and molecular species composition in soft tissues of Crassostrea lugubris were investigated for the first time from Vietnam. Phosphatidylglycolic acid (PGA) is the new phospholipid class first identified in marine species in general and Crassostrea lugubris in particular. Main eight classes of PL were determined in PoL fraction: diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylserine (PS), ceramide aminoethylphosphonate (CAEP), CAEP with hydroxylated FAs (CAEP-OH), and lysophosphatidylcholine. PE and PC accounted for approximately 63% of total known PL. Polyunsaturated FAs accounted for more than 30% of TL. Ninety molecular species of glycerophospholipids, including PGA, PE, PC, PS, PI, DPG, and PG, and sphingophosphonolipids (CAEP) were identified in PoL. Alkenyl-acyl forms of glycerophospholipids were predominated in the molecular species of PGA, PE, and PS. PGA 38:1 (p18:0/20:1), PE 40:6 (p18:0/22:6 and p18:1/22:5), PC 30:0 (14:0/16:0), PS 38:1 (p18:0/20:1), PI 40:5 (20:1/20:4), PG 32:0 (16:0/16:0), DPG 88:24 (22:6/22:6/22:6/22:6), and CAEP 34:2 (d18:2/16:0) were major molecular species.

Keywords: Crassostrea lugubris; fatty acids; high‐resolution mass spectrometry; lipid classes; molecular species; oyster; phosphatidylglycolic acid; phospholipids.