FOXO1 and ANGPT2 relative gene expression in non-ST-segment elevation myocardial infarction among patients with or without type 2 diabetes

Tomasz Skowerski; Katarzyna Nabrdalik; Hanna Kwiendacz; Maciej Pajak; Andrzej Ochała; Katarzyna Mizia-Stec; Zbigniew Gąsior; Janusz Gumprecht

doi:10.5114/aic.2021.107498

FOXO1 and ANGPT2 relative gene expression in non-ST-segment elevation myocardial infarction among patients with or without type 2 diabetes

Postepy Kardiol Interwencyjnej. 2021 Jun;17(2):187-192. doi: 10.5114/aic.2021.107498. Epub 2021 Jul 9.

Authors

Tomasz Skowerski^{1

2}, Katarzyna Nabrdalik², Hanna Kwiendacz², Maciej Pajak³, Andrzej Ochała⁴, Katarzyna Mizia-Stec⁵, Zbigniew Gąsior¹, Janusz Gumprecht²

Affiliations

¹ Department of Cardiology, School of Health Sciences, Medical University of Silesia, Katowice, Poland.
² Department of Internal Medicine, Diabetology and Nephrology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, Poland.
³ Roslin Institute, Midlothian, Edinburgh, United Kingdom of Great Britain and Northern Ireland.
⁴ Division of Cardiology and Structural Heart Diseases Medical University of Silesia, Katowice, Poland.
⁵ First Department of Cardiology, Faculty of Medical Sciences, Medical University of Silesia, Katowice, Poland.

Abstract

Introduction: It is well known that chronic hyperglycemia or chronic inflammation leads to both FOXO1 and Ang-2 gene (ANGPT2) expression induction in endothelial cells. ANGPT2 and FOXO1 relative gene expression in peripheral blood cells in diabetes and myocardial ischemia were not researched extensively.

Aim: Our objective was to evaluate ANGPT2 and FOXO1 gene expression in peripheral blood cells in patients with non-ST elevation myocardial infarction (NSTEMI), both with and without type 2 diabetes mellitus (T2DM), and compare them to the results obtained from T2DM and control subjects.

Material and methods: This was a multi-center, prospective study of 138 NSTEMI patients with/without T2DM, T2DM and a control group. FOXO1, ANGPT2, TBP (TATA box binding protein - as a reference gene) gene expression levels in peripheral blood cells were measured in each patient. Electrocardiography and echocardiography with assessment of ejection fraction (EF) were performed. Patients with NSTEMI underwent urgent (< 24 h) coronarography and the SYNTAX score and GRACE 2.0 score were calculated.

Results: The ANGPT2 gene relative expression in buffy coat in the analyzed samples was very low and detectable only in 11 patients from all groups (8.66% of all patients). The level of FOXO1 gene relative expression was significantly higher in patients with NSTEMI (median relative expression = 1.39) than in non-NSTEMI patients (median = 1.09) (W = 1578, p < 0.05) regardless of the presence of T2DM. The FOXO1 gene relative expression was not correlated with GRACE 2.0 score or SYNTAX score of NSTEMI patients. We did not observe any significant change in FOXO1 gene expression after successful angioplasty.

Conclusions: On the basis of our results we can conclude that analyzing the ANGPT2 gene relative expression in peripheral blood cells has no role in assessment of CAD complexity among patients with and without T2DM. FOXO1 gene relative expression in blood peripheral cells is elevated in patients with NSTEMI regardless of the presence of T2DM. FOXO1 expression does not decrease after successful percutaneous coronary intervention and is not correlated with the severity of CAD in patients with NSTEMI.

Keywords: angiopoietin-2; diabetes; foxo-1; non-ST elevation myocardial infarction.