Measuring the Rate of In-vitro Drug Release From Polymeric Nanoparticles by 19F Solution State NMR Spectroscopy

Abstract

We report what we believe is the first use of ¹⁹F NMR spectroscopy to directly measure in-vitro release (IVR) from polymeric nanoparticles (PNPs). Using ¹⁹F NMR we selectively measured IVR of AZD2811 from PNPs. Due to rapid nuclear relaxation in solid-like environments only AZD2811 in solution is detected, and physical separation from the PNPs isn't required. The NMR approach and ultra-centrifugation/UHPLC were shown to be equivalent. The selectivity of ¹⁹F NMR means it is readily applied to complex IVR media such as recombinant human serum albumin (rHSA).

Keywords: Analytical chemistry; NMR; Nanomedicines; Nanoparticles; PLA.