Phenolic Profiling and Therapeutic Potential of Certain Isolated Compounds from Parkia roxburghii against AChE Activity as well as GABAA α5, GSK-3β, and p38α MAP-Kinase Genes

Heba A El Gizawy; Heba M Abo-Salem; Ali A Ali; Mohammed A Hussein

doi:10.1021/acsomega.1c02340

Phenolic Profiling and Therapeutic Potential of Certain Isolated Compounds from Parkia roxburghii against AChE Activity as well as GABA_A α5, GSK-3β, and p38α MAP-Kinase Genes

ACS Omega. 2021 Jul 26;6(31):20492-20511. doi: 10.1021/acsomega.1c02340. eCollection 2021 Aug 10.

Authors

Heba A El Gizawy¹, Heba M Abo-Salem², Ali A Ali³, Mohammed A Hussein⁴

Affiliations

¹ Pharmacognosy Department, Faculty of Pharmacy, October 6 University, 12585 6th of October City, Egypt.
² Chemistry and Natural Compounds Department, Pharmaceutical and Drug Industries Research Division, National Research Center, Dokki, 12585 Giza, Egypt.
³ Postgraduate Studies, October 6 University, 12585 Sixth of October City, Egypt.
⁴ Biochemistry Department, Faculty of Applied Medical Sciences, October 6 University, 12585 Sixth of October City, Egypt.

Abstract

Parkia roxburghii belongs to the family Mimosaceae; it has been used since ancient times as a cure for different health complications; such as inflammatory and gynecological diseases and hemiplegia. In this investigation, a reversed-phase-high-performance liquid chromatography (RP-HPLC) profile was carried out for P. roxburghii; also, the isolated bioactive compounds including quercetin, catechin, and biochaninA were individually and/or in combination investigated for their inhibitory effects on scopolamine-induced memory impairments in mice, implying that they have the ability to reduce the neurodegenerative effects of scopolamine and thus could be employed as a more effective therapeutic agent in the treatment of Alzheimer's disease (AD) in humans. The possible interactions of Parkia flavonoids with acetylcholinesterase (AChE), γ-aminobutyric acid A receptor, alpha5 (GABA_A α5), glycogen synthase kinase-3 (GSK-3), p38 mitogen-activated protein kinase (p38MAP-kinase), signal-regulated kinase (ERK), and protein-serine/threonine kinase (Akt) were then determined using molecular docking.