BMV Propagation, Extraction and Purification Using Chromatographic Methods

Aleksander Strugała; Paulina Bierwagen; Jakub Dalibor Rybka; Michał Giersig; Marek Figlerowicz; Anna Urbanowicz

doi:10.21769/BioProtoc.2935

BMV Propagation, Extraction and Purification Using Chromatographic Methods

Bio Protoc. 2018 Jul 20;8(14):e2935. doi: 10.21769/BioProtoc.2935.

Authors

Aleksander Strugała¹, Paulina Bierwagen¹, Jakub Dalibor Rybka², Michał Giersig^{2

3}, Marek Figlerowicz^{1

4

5}, Anna Urbanowicz^{1

6}

Affiliations

¹ Institute of Bioorganic Chemistry, Polish Academy of Science, Poznań, Poland.
² Center of Advanced Technologies, Adam Mickiewicz University, Poznań, Poland.
³ Institut für Experimentalphysik, Freie Universität Berlin, Berlin, Germany.
⁴ European Center for Bioinformatics and Genomics, Poznań, Poland.
⁵ Institute of Computing Science, Poznan University of Technology, Poznań, Poland.
⁶ Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland.

Abstract

Brome mosaic virus (BMV) is a well-known plant virus representing single-stranded RNA (ssRNA) positive-sense viruses. It has been widely used as a model in multiple studies concerning plant virus biology, epidemiology and the application of viral capsids in nanotechnology. Herein, we describe a method for BMV purification based on ion-exchange and size-exclusion chromatography. The presented method is of similar efficiency to previously described protocols relying on differential centrifugation and can easily be scaled up. The resulting BMV capsids are stable and monodisperse and can be used for further applications.

Keywords: BMV; Brome mosaic virus; Capsid; Plant virus purification; Virion; Virus-like particles (VLP).