Current advances in overcoming obstacles of CRISPR/Cas9 off-target genome editing

Guillermo Aquino-Jarquin

doi:10.1016/j.ymgme.2021.08.002

Current advances in overcoming obstacles of CRISPR/Cas9 off-target genome editing

Mol Genet Metab. 2021 Sep-Oct;134(1-2):77-86. doi: 10.1016/j.ymgme.2021.08.002. Epub 2021 Aug 8.

Author

Guillermo Aquino-Jarquin¹

Affiliation

¹ Laboratorio de Investigación en Genómica, Genética y Bioinformática, Hospital Infantil de México, Federico Gómez, Ciudad de México, Mexico; Departamento de Ciencias Naturales, Unidad Cuajimalpa, Universidad Autónoma Metropolitana, Ciudad de México, Mexico. Electronic address: guillaqui@himfg.edu.mx.

PMID: 34391646
DOI: 10.1016/j.ymgme.2021.08.002

Abstract

CRISPR/Cas9-based technology has revolutionized biomedical research by providing a high-fidelity gene-editing method, foreshadowing a significant impact on the therapeutics of many human genetic disorders previously considered untreatable. However, off-target events represent a critical hurdle before genome editing can be fully established in clinical practice. This mini-review recapitulates some recent advances for detecting and overcoming off-target effects mediated by the CRISPR/Cas9 system that could increase the likelihood of clinical success of the CRISPR-based approaches.

Keywords: Anti-CRISPR proteins; Base editors; CRISPR/Cas9; Genome-editing; Off-target effects; Prime editing.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

CRISPR-Cas Systems / genetics*
Gene Editing / methods*
Gene Editing / standards
Humans