Environmental heterogeneity determines the response patterns of microbially mediated N-reduction processes to sulfamethoxazole in river sediments

Abstract

The widespread occurrence of antibiotics in aquatic ecosystems leads to potential ecological risks to organisms, in turn affecting microbially mediated processes. Here, we investigated the response of dominant N-reduction processes to the frequently detected antibiotic sulfamethoxazole (SMX) along the Chaobai River with regional environmental heterogeneity, including denitrification, anaerobic ammonium oxidation (anammox), dissimilatory nitrate reduction to ammonium (DNRA), and nitrous oxide (N₂O) release. We found two divergent SMX response patterns for denitrification in contrasting scenarios of geochemical properties. In the context of low nitrate and carbon, SMX weakened denitrification with a slightly stimulation first. Whereas SMX directly inhibited denitrification when nitrate and carbon were sufficient. High SMX concentration suppressed anammox (26-72%) and DNRA activities (48-84%) via restraining the activities of anammox and DNRA bacteria. Notably, SMX increased the contribution of denitrification to N-reduction at the expense of DNRA to N-reduction, leading to a shift in nitrogen conversion towards denitrification. Additionally, SMX stimulated N₂O emission (up to 91%) due to superior restraint on process of N₂O reduction to N₂ and an incline for N-reduction towards denitrification, thereby exacerbating greenhouse effect. Our results advance the understanding of how nitrogen cycling is affected by SMX in aquatic ecosystems with environmental heterogeneity.

Keywords: Anammox; DNRA; Denitrification; Functional genes; Microbial community.