Innovative mycoremediation technique for treating unsterilized PCDD/F-contaminated field soil and the exploration of chlorinated metabolites

Acharee Kaewlaoyoong; Jenq-Renn Chen; Chih-Yu Cheng; Chitsan Lin; Nicholas Kiprotich Cheruiyot; Pongsert Sriprom

doi:10.1016/j.envpol.2021.117869

Innovative mycoremediation technique for treating unsterilized PCDD/F-contaminated field soil and the exploration of chlorinated metabolites

Environ Pollut. 2021 Nov 15:289:117869. doi: 10.1016/j.envpol.2021.117869. Epub 2021 Aug 6.

Authors

Acharee Kaewlaoyoong¹, Jenq-Renn Chen², Chih-Yu Cheng³, Chitsan Lin⁴, Nicholas Kiprotich Cheruiyot⁵, Pongsert Sriprom⁶

Affiliations

¹ Department of Safety, Health and Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, 82445, Taiwan; Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, 807, Taiwan.
² Department of Safety, Health and Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, 82445, Taiwan.
³ Department of Marine Biotechnology, National Kaohsiung University of Science and Technology, Kaohsiung, 81157, Taiwan.
⁴ Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, 81157, Taiwan. Electronic address: ctlin@nkust.edu.tw.
⁵ Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, 81157, Taiwan.
⁶ Program of Food Process Engineering, Faculty of Food Industry, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.

PMID: 34388555
DOI: 10.1016/j.envpol.2021.117869

Abstract

Mycoremediation of unsterilized PCDD/F-contaminated field soil was successfully demonstrated by solid-state fermentation coupled with Pleurotus pulmonarius utilizing a patented incubation approach. The experiments were carried out in four setups with two as controls. The contaminated soil was homogenously mixed with solid inocula, 1:0.5 dry w/w, resulting in an initial concentration of 4432 ± 623 ng WHO-TEQ kg^-1. After a 30-day incubation under controlled conditions, the overall removal (approx. 60%) was non-specific. The removal was attributed to degradation by extracellular ligninolytic enzymes and uptake into the fruiting tissue (~110 ng WHO-TEQ kg^-1 of mushroom). Furthermore, less recalcitrant chlorinated metabolites were found, implying ether bond cleavage and dechlorination happened during the mycoremediation. These metabolites resulted from the complex interaction between P. pulmonarius and the indigenous microbes from the unsterilized soil. This study provides a new step toward scaling up this mycoremediation technique to treat unsterilized PCDD/F-contaminated field soil.

Keywords: Biodegradation; Pleurotus pulmonarius; Solid-state fermentation; Uptake of PCDD/Fs; White rot fungus.

MeSH terms

Benzofurans* / analysis
Dibenzofurans, Polychlorinated
Pleurotus
Polychlorinated Dibenzodioxins* / analysis
Soil
Soil Pollutants* / analysis

Substances

Benzofurans
Dibenzofurans, Polychlorinated
Polychlorinated Dibenzodioxins
Soil
Soil Pollutants

Supplementary concepts

Pleurotus pulmonarius