Bioconjugation of Peptides to Hybrid Gold Nanoparticles

Abstract

Gold nanoparticles (AuNPs) can be produced by well-assessed synthesis methods and can show a high surface area-to-volume ratio, chemical inertness, high electron density, strong optical absorption as well as low toxicity. AuNPs have been conjugated with many different biomolecules for a wide range of biomedical applications. These applications require an increasingly complex level of surface decoration in order to achieve stability, efficacy, and specific functionalities. This chapter provides detailed instructions about the synthesis of AuNPs and bioconjugation strategies in order to obtain stable hybrid nanomaterials. The described biofunctionalization procedures are based on carbodiimide chemistry and ligand-exchange methods allowing the conjugation of Lys-peptide or Cys-peptide, respectively, to the AuNPs surface.

Keywords: Bioconjugation; Covalent conjugation; Gold nanoparticles (AuNPs) peptides; Surface functionalization.