Genetic plasticity in RNA2 is associated with pathogenic diversification of broad bean wilt virus 2

Myung-Hwi Kim; Hae-Ryun Kwak; Boram Choi; Sun-Jung Kwon; Jang-Kyun Seo

doi:10.1016/j.virusres.2021.198533

Genetic plasticity in RNA2 is associated with pathogenic diversification of broad bean wilt virus 2

Virus Res. 2021 Oct 15:304:198533. doi: 10.1016/j.virusres.2021.198533. Epub 2021 Aug 9.

Authors

Myung-Hwi Kim¹, Hae-Ryun Kwak², Boram Choi³, Sun-Jung Kwon³, Jang-Kyun Seo⁴

Affiliations

¹ Department of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of Korea; Integrated Major in Global Smart Farm, Seoul National University, Seoul 08826, Republic of Korea.
² Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea.
³ Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea.
⁴ Department of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Republic of Korea; Integrated Major in Global Smart Farm, Seoul National University, Seoul 08826, Republic of Korea; Institutes of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Republic of Korea. Electronic address: jangseo@snu.ac.kr.

PMID: 34384805
DOI: 10.1016/j.virusres.2021.198533

Abstract

Broad bean wilt virus 2 (BBWV2) is an evolutionarily successful RNA virus with an extensive host range and worldwide distribution that causes severe damage to crops. While numerous BBWV2 isolates from various plant species have been identified and their genome sequences determined, little information is available on the virulence and symptomatic characteristics corresponding to the genomic sequences. In this study, we provide integrated information on the molecular and pathogenic characteristics of three genetically distant BBWV2 isolates: BBWV2-PC, -LS2, and P3 obtained from Gentiana scabra, Leonurus sibiricus, and Pisum sativum, respectively. Phylogenetic and diversity analyses of the BBWV2 population included 42 isolates from various host species and revealed that RNA2 has higher genetic plasticity than RNA1 and may have evolved under host-imposed constraints. In addition, we generated an infectious cDNA clone of BBWV2-PC RNA2 (pBBWV2-PC-R2). Pseudo-recombination analysis of pBBWV2-PC-R2 further demonstrated that RNA2 determines the pathogenic characteristics of the PC isolate.

Keywords: Broad bean wilt virus 2; Pathogenicity; Population diversity; Symptomatic variation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fabavirus*
Phylogeny
RNA, Viral / genetics

Substances

RNA, Viral

Supplementary concepts

Broad bean wilt virus 2