Development of an anti-infective coating on the surface of intraosseous implants responsive to enzymes and bacteria

Abstract

Background: Bacterial proliferation on the endosseous implants surface presents a new threat to the using of the bone implants. Unfortunately, there is no effective constructed antibacterial coating which is bacterial anti-adhesion substrate-independent or have long-term biofilm inhibition functions.

Methods: Drug release effect was tested in Chymotrypsin (CMS) solution and S. aureus. We used bacterial inhibition rate assays and protein leakage experiment to analyze the in vitro antibacterial effect of (Montmorillonite/Poly-L-lysine-Chlorhexidine)₁₀ [(MMT/PLL-CHX)₁₀] multilayer film. We used the CCK-8 assay to analyze the effect of (MMT/PLL-CHX)₁₀ multilayer films on the growth and proliferation of rat osteoblasts. Rat orthopaedic implant-related infections model was constructed to test the antimicrobial activity effect of (MMT/PLL-CHX)₁₀ multilayer films in vivo.

Results: In this study, the (MMT/PLL-CHX)₁₀ multilayer films structure were progressively degraded and showed well concentration-dependent degradation characteristics following incubation with Staphylococcus aureus and CMS solution. Bacterial inhibition rate assays and protein leakage experiment showed high levels of bactericidal activity. While the CCK-8 analysis proved that the (MMT/PLL-CHX)₁₀ multilayer films possess perfect biocompatibility. It is somewhat encouraging that in the in vivo antibacterial tests, the K-wires coated with (MMT/PLL-CHX)₁₀ multilayer films showed lower infections incidence and inflammation than the unmodified group, and all parameters are close to SHAM group.

Conclusion: (MMT/PLL-CHX)₁₀ multilayer films provides a potential therapeutic method for orthopaedic implant-related infections.

Keywords: Antibacterial effect; Infections; Intraosseous implants; Microenvironment; Multilayer films.