Microbially induced carbonate precipitation (MICP) is an advanced bioremediation approach to remediate heavy metal (HM)-contaminated water and soil. In this study, metal-tolerant urease-producing bacterial isolates, namely, UR1, UR16, UR20, and UR21, were selected based on their urease activity. The efficiency of these isolates in water for Pb and Cd immobilizations was explored. Our results revealed that UR21 had the highest removal rates of Pb (81.9%) and Cd (65.0%) in solution within 72 h through MICP. The scanning electron microscopy-energy-dispersive x-ray and x-ray diffraction analysis confirmed the structure and the existence of PbCO3 and CdCO3 crystals in the precipitates. In addition, the strain UR21, in combination with urea/eggshell waste (EGS) or both, was further employed to investigate the effect of MICP on soil enzymatic activity, chemical fractions, and bioavailability of Pb and Cd. The outcomes indicated that the applied treatments reduced the proportion of soluble-exchangeable-Pb and -Cd, which resulted in an increment in carbonate-bound Pb and Cd in the soil. The DTPA-extractable Pb and Cd were reduced by 29.2% and 25.2% with the treatment of UR21+urea+EGS as compared to the control. Besides, the application of UR21 and EGS significantly increased the soil pH, cation exchange capacity, and enzyme activities. Our findings may provide a novel perceptive for an eco-friendly and sustainable approach to remediate heavy metal-contaminated environment through a combination of metal-resistant ureolytic bacterial strain and EGS.
Keywords: Bioremediation; Cadmium; Eggshell waste; Lead; Microbially induced carbonate precipitation; Ureolytic bacteria.
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.