The first case of Spiroxys contortus in European pond turtle (Emys orbicularis) in the wild in Poland

Marta Demkowska-Kutrzepa; Klaudiusz Szczepaniak; Monika Roczeń-Karczmarz; Ezequiel O Palumbo; Maria Studzińska; Paweł Różański; Krzysztof Tomczuk

doi:10.1016/j.ijppaw.2021.07.004

The first case of Spiroxys contortus in European pond turtle (Emys orbicularis) in the wild in Poland

Int J Parasitol Parasites Wildl. 2021 Jul 15:16:26-29. doi: 10.1016/j.ijppaw.2021.07.004. eCollection 2021 Dec.

Authors

Marta Demkowska-Kutrzepa¹, Klaudiusz Szczepaniak¹, Monika Roczeń-Karczmarz¹, Ezequiel O Palumbo², Maria Studzińska¹, Paweł Różański³, Krzysztof Tomczuk¹

Affiliations

¹ Department of Parasitology and Invasive Diseases. Faculty of Veterinary Medicine University of Life Sciences in Lublin, Poland.
² Centro de Estudios Parasitológicos y de Vectores (CEPAVE) CCT LA PLATA (CONICET-UNLP), Buenos Aires, Argentina.
³ Department of Animal and Environmental Hygiene. Faculty of Animal Breeding and Biology, University of Life Sciences in Lublin, Poland.

Abstract

During standard parasitological dissection of 4 individuals of European pond turtle (E. orbicularis) (Linneaus, 1758) one nematode was found. In a morphological examination the parasite was identified as Spiroxys contortus (Rudolphi, 1819) and confirmed by DNA analysis based on sequencing of the small subunit ribosomal RNA gene. The partial 18S rDNA gene was deposited to NCBI GenBank with the accession number MN629917. This is the first molecular evidence of S. contortus in E. orbicularis from wild from Poland. The analyzed sample genotype sequence shows 100% similarity to the reference specimen from Argentina.

Keywords: DNA sequence; Emys orbicularis; Poland; Spiroxys contortus.