Characterization, Identification and Evaluation of Wheat- Aegilops sharonensis Chromosome Derivatives

Xiaolu Wang; Zhihui Yu; Hongjin Wang; Jianbo Li; Ran Han; Wenjing Xu; Guangrong Li; Jun Guo; Yan Zi; Faji Li; Dungong Cheng; Aifeng Liu; Haosheng Li; Zujun Yang; Jianjun Liu; Cheng Liu

doi:10.3389/fpls.2021.708551

Characterization, Identification and Evaluation of Wheat- Aegilops sharonensis Chromosome Derivatives

Front Plant Sci. 2021 Jul 26:12:708551. doi: 10.3389/fpls.2021.708551. eCollection 2021.

Authors

Xiaolu Wang^{1

2

3}, Zhihui Yu⁴, Hongjin Wang⁴, Jianbo Li^{1

2

3}, Ran Han^{1

2

3}, Wenjing Xu^{1

2

3}, Guangrong Li⁴, Jun Guo^{1

2

3}, Yan Zi^{1

2

3}, Faji Li^{1

2

3}, Dungong Cheng^{1

2

3}, Aifeng Liu^{1

2

3}, Haosheng Li^{1

2

3}, Zujun Yang⁴, Jianjun Liu^{1

2

3}, Cheng Liu^{1

2

3}

Affiliations

¹ Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, China.
² Key Laboratory of Wheat Biology and Genetic Improvement in the North Huang and Huai River Valley, Ministry of Agriculture, Jinan, China.
³ National Engineering Laboratory for Wheat and Maize, Jinan, China.
⁴ School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chengdu, China.

Abstract

Aegilops sharonensis, a wild relative of wheat, harbors diverse disease and insect resistance genes, making it a potentially excellent gene source for wheat improvement. In this study, we characterized and evaluated six wheat-A. sharonensis derivatives, which included three disomic additions, one disomic substitution + monotelosomic addition and two disomic substitution + disomic additions. A total of 51 PLUG markers were developed and used to allocate the A. sharonensis chromosomes in each of the six derivatives to Triticeae homoeologous groups. A set of cytogenetic markers specific for A. sharonensis chromosomes was established based on FISH using oligonucleotides as probes. Molecular cytogenetic marker analysis confirmed that these lines were a CS-A. sharonensis 2S^sh disomic addition, a 4S^sh disomic addition, a 4S^sh (4D) substitution + 5S^shL monotelosomic addition, a 6S^sh disomic addition, a 4S^sh (4D) substitution + 6S^sh disomic addition and a 4S^sh (4D) substitution + 7S^sh disomic addition line, respectively. Disease resistance investigations showed that chromosome 7S^sh of A. sharonensis might harbor a new powdery mildew resistance gene, and therefore it has potential for use as resistance source for wheat breeding.

Keywords: Aegilops sharonensis; PLUG marker; chromosome derivatives; cytogenetic identification; powdery mildew resistance.