Dissolved oxygen disturbs nitrate transformation by modifying microbial community, co-occurrence networks, and functional genes during aerobic-anoxic transition

Abstract

No consensus has been achieved among researchers on the effect of dissolved oxygen (DO) on nitrate (NO₃^--N) transformation and the microbial community, especially during aerobic-anoxic transition. To supplement this knowledge, NO₃^--N transformation, microbial communities, co-occurrence networks, and functional genes were investigated during aerobic-anoxic transition via microcosm simulation. NO₃^--N transformation rate in the early stage (DO ≥2 mg/L) was always significantly higher than that in the later stage (DO <2 mg/L) during aerobic-anoxic transition, and NO₂^--N accumulation was more significant during the anoxic stage, consistent with the result obtained under constant DO conditions. These NO₃^--N transformation characteristics were not affected by other environmental factors, indicating the important role of DO in NO₃^--N transformation during aerobic-anoxic transition. Changes in DO provoked significant alterations in microbial diversity and abundance of functional bacteria dominated by Massilia, Bacillus, and Pseudomonas, leading to the variation in NO₃^--N transformation. Co-occurrence network analysis revealed that NO₃^--N transformation was performed by the interactions between functional bacteria including symbiotic and competitive relationship. In the presence of oxygen, these interactions accelerated the NO₃^--N transformation rate, and bacterial metabolization proceeded via increasingly varied pathways including aerobic and anoxic respiration, which was demonstrated through predicted genes. The higher relative abundance of genes narG, narH, and napA suggested the occurrence of coupled aerobic-anoxic denitrification in the early stage. NO₃^--N transformation rate decreased accompanied by a significant NO₂^--N accumulation with the weakening of coupled aerobic-anoxic denitrification during aerobic-anoxic transition. Structural equation modeling further demonstrated the relationship between DO and NO₃^--N transformation. DO affects NO₃^--N transformation by modifying microbial community, bacterial co-occurrence, and functional genes during aerobic-anoxic transition.

Keywords: Aerobic-anoxic transition; Co-occurrence network; Dissolved oxygen; Microbial communities; Nitrate transformation.