Fast screening test for molecular recognition of levodopa and dopamine in biological samples using 3D printed stochastic microsensors

Abstract

The simultaneous assay of levodopa and dopamine is essential for diagnosis and treatment of neurodegenerative diseases and brain cancer. 3D stochastic microsensors based on multi-walled carbon nanotubes (MWCNTs), gold nanoparticles (AuNPs) and 1-adamantyloleamide (AOA) was used for the simultaneous molecular recognition of levodopa and dopamine in biological samples (whole blood, urine, and brain tissue). The proposed 3D stochastic microsensors presented low limits of quantification, and high sensitivities. High selectivity was recorded versus neurotransmitters such as epinephrine, norepinephrine, serotonin, and glutamate. High recoveries were obtained for the assay of both levodopa and dopamine in whole blood, urine, and tumor tissue samples.

Keywords: 3D printed stochastic sensors; Brain cancer; Dopamine; Levodopa.