Advanced fluorescence correlation spectroscopy for studying biomolecular conformation

Arindam Ghosh; Jörg Enderlein

doi:10.1016/j.sbi.2021.06.018

Advanced fluorescence correlation spectroscopy for studying biomolecular conformation

Curr Opin Struct Biol. 2021 Oct:70:123-131. doi: 10.1016/j.sbi.2021.06.018. Epub 2021 Aug 6.

Authors

Arindam Ghosh¹, Jörg Enderlein²

Affiliations

¹ Third Institute of Physics, Biophysics, University of Göttingen, Friedrich Hund Platz 1 Göttingen, 37077, Germany.
² Third Institute of Physics, Biophysics, University of Göttingen, Friedrich Hund Platz 1 Göttingen, 37077, Germany; Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), Georg August University, Göttingen, 37077, Germany. Electronic address: jenderl@gwdg.de.

PMID: 34371261
DOI: 10.1016/j.sbi.2021.06.018

Abstract

We present the recent developments and advances in fluorescence correlation spectroscopy (FCS) and their application to the investigation of biomolecular conformations. In particular, we present and discuss three techniques: multichannel nanosecond FCS, photo-induced electron transfer FCS, and fluorescence lifetime correlation spectroscopy. We briefly describe each method and discuss recent applications to diverse biophysical studies of biomolecular conformation.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Molecular Conformation
Spectrometry, Fluorescence*