Objective: To observe the influence of electroacupuncture(EA) combined with aconitine on the hemodyna-mics, echocardiogram, and arrhythmias in heart failure rats, so as to explore the facilitation and attenuation effects of EA combined with aconitine.
Methods: SD rats were randomly divided into control, model, aconitine and aconitine+EA groups, with 6 rats in each group. Propranolol hydrochloride was used to establish the heart failure model. Rats in the aconitine group were trea-ted with aconitine continuously for 1 h (40 μg/kg). Rats in the aconitine +EA group were given the same treatment as the aconitine group, meanwhile, EA (3 mA, 2 Hz/15 Hz) was applied at "Neiguan"(PC6) for 30 min. Left ventricular catheter and small animal ultrasound imaging system were used to observe the heart hemodynamic indexes such as left ventricular systolic pressure(LVSP), maximal rate for left ventricular pressure rising (+dp/dtmax), and maximal rate for left ventricular pressure declining (-dp/dtmax), ejection fraction (EF) and fractional shortening (FS). The incidence rate of arrhythmia and arrhythmia score was observed by electrocardiogram.
Results: Following modeling and compared with the control group, LVSP, +dp/dtmax, -dp/dtmax, EF and FS in the aconitine group all decreased(P<0.01) and maintained in the model group. The LVSP of rats in the aconitine group was higher than that of the model group at 15 min after administration of aconitine (P<0.05), and +dp/dtmax was higher at 15, 60 min after administration (P<0.05). Since 15 min after administration, EF and FS in the aconitine group were significantly higher than those of the model group (P<0.01, P<0.05). After EA intervention, compared with the aconitine group, LVSP, +dp/dtmax, -dp/dtmax in the aconitine+EA group were significantly increased (P<0.01, P<0.05) during administration and EF and FS in the aconitine+EA group significantly increased at the beginning of administration of aconitine and 30 and 60 min during administration (P<0.05, P<0.01). The incidence rate of arrhythmia was 100% in the aconitine group, and 50.0% in the rats of aconitine + EA group. The arrhythmia score of aconitine + EA group was significantly lower than that of aconitine group (P<0.05).
Conclusion: Aconitine has a certain inotropic effect, but it is easy to cause arrhythmia. The combination of EA and aconitine can not only improve the contractile function of the heart in rats with heart failure, but also reduce the toxic reaction of aconitine.
目的：观察电针联合乌头碱对心力衰竭大鼠血流动力学、超声心动图及心律失常情况的影响，探讨其增效减毒效应。方法：SD大鼠随机分为对照组、模型组、乌头碱组及乌头碱+电针组，每组6只。采用股静脉注射盐酸普萘洛尔复制心力衰竭大鼠模型。造模成功10 min后，乌头碱组予以乌头碱持续给药治疗1 h(40 μg/kg)；乌头碱+电针组在乌头碱组治疗的基础上同时给予电针“内关”治疗(3 mA，2 Hz/15 Hz，30 min)。采用左心室导管、小动物超声成像系统观察造模前、造模后、给药即刻和给药后15、30、60 min时左室内压(LVSP)、左室内压最大上升速率(+dp/dtmax)、左室内压最大下降速率(-dp/dtmax)、左室射血分数(EF)、左室短轴缩短率(FS)的变化；通过心电图观察比较各组大鼠心律失常情况。结果：与对照组比较，造模后模型组大鼠LVSP、+dp/dtmax、-dp/dtmax、EF、FS均明显下降且能维持至实验结束(P<0.01)。乌头碱组大鼠给药15 min时，LVSP高于模型组(P<0.05)；给药15、60 min时，+dp/dtmax高于模型组(P<0.05)；给药15 min起，各时点EF、FS均明显高于模型组(P<0.05，P<0.01)。与乌头碱组比较，在给药各个时间点，乌头碱+电针组LVSP、+dp/dtmax、-dp/dtmax均明显升高(P<0.01，P<0.05)，EF、FS在给药即刻、30 min、60 min时明显升高(P<0.01，P<0.05)。乌头碱+电针组大鼠心律失常发生率为50.0%，明显低于乌头碱组(100%，P<0.05)；乌头碱+电针组大鼠心律失常评分明显低于乌头碱组(P<0.05)。结论：乌头碱具有一定的强心作用，但易导致心律失常的发生。而电针与乌头碱联用，不仅可以更明显地改善心力衰竭大鼠心脏收缩功能，且可以减小乌头碱的毒性反应。.
Keywords: Aconitine; Echocardiogram; Electroacupuncture; Facilitation and attenuation effect; Heart failure; Hemodynamics.