Phylogenetic relationship and characterization of the complete mitochondrial genome of Cheilomenes sexmaculata (Coleoptera: Coccinellidae)

Gaoqi Cheng; Yimin Du; Xinjun Liu

doi:10.1080/23802359.2021.1955767

Phylogenetic relationship and characterization of the complete mitochondrial genome of Cheilomenes sexmaculata (Coleoptera: Coccinellidae)

Mitochondrial DNA B Resour. 2021 Jul 21;6(8):2449-2451. doi: 10.1080/23802359.2021.1955767. eCollection 2021.

Authors

Gaoqi Cheng^{1

2}, Yimin Du^{1

2}, Xinjun Liu^{1

2}

Affiliations

¹ School of Life Sciences, Gannan Normal University, Ganzhou, China.
² National Navel Orange Engineering and Technology Research Center, Ganzhou, China.

Abstract

Cheilomenes sexmaculata is a common natural enemy for aphid and psyllid in agricultural systems in South China. In this study, we sequenced and analyzed the complete mitochondrial genome (mitogenome) of C. sexmaculata. This mitogenome was 17,297 bp long and encoded 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs) and two ribosomal RNA unit genes (rRNAs). Gene order was conserved and identical to most other previously sequenced Coccinellidae. All PCGs of C. sexmaculata have the conventional start codon for invertebrate mitochondrial PCGs (ATN), with the exception of cox1 (AAT) and nad3 (TTG). Except for seven genes (cox1, cox2, cox3, nad3, nad5, nad4 and nad6) end with the incomplete stop codon T-, all other PCGs terminated with the stop codon TAA or TAG. The whole mitogenome exhibited heavy AT nucleotide bias (78.0%). Phylogenetic analysis positioned C. sexmaculata in a well-supported clade with Aiolocaria hexaspilota. The relationships (Sticholotidinae + (Coccinellinae + (Scymninae + Epilachninae))) were supported in Coccinellidae, and Halyziini was paraphyletic to Coccinellini within Coccinellinae.

Keywords: Cheilomenes sexmaculata; Ladybird; mitochondrial genome; phylogenetic analysis.

Grants and funding

This study was supported by the Major Science and Technology R&D Program of Jiangxi Province [20194ABC28007], Foundation of Jiangxi Province Educational Committee [GJJ180779] and the National Key R&D Program of China [2018YFD0201504].