Determination of Alkaloids and Flavonoids in Sophora flavescens by UHPLC-Q-TOF/MS

Yaqian Dong; Guoxiang Jia; Jingwen Hu; Hui Liu; Tingting Wu; Shenshen Yang; Yubo Li; Ting Cai

doi:10.1155/2021/9915027

Determination of Alkaloids and Flavonoids in Sophora flavescens by UHPLC-Q-TOF/MS

J Anal Methods Chem. 2021 Jul 28:2021:9915027. doi: 10.1155/2021/9915027. eCollection 2021.

Authors

Yaqian Dong¹, Guoxiang Jia¹, Jingwen Hu¹, Hui Liu¹, Tingting Wu¹, Shenshen Yang¹, Yubo Li¹, Ting Cai^{2

3}

Affiliations

¹ School of Traditional Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, No. 10 Poyang Lake Road, Tuanbo New City, Jinghai District, Tianjin 301617, China.
² Hwa Mei Hospital, University of Chinese Academy of Sciences (Ningbo No. 2 Hospital), Ningbo 315010, China.
³ Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, Ningbo 315010, China.

Abstract

This study is based on UHPLC-Q-TOF/MS and fragment ions to achieve classification and identification of alkaloids and flavonoids in Sophora flavescens. By reviewing the available and relevant literature, the mass fragmentation rules of alkaloids and flavonoids were summarized. 0.1% formic acid water (A) and acetonitrile (B) were used as mobile phases. 37 chemical constituents were identified, including 13 alkaloids and 24 flavonoids. This research method offers a complete strategy based on the fragmentation information of characteristic fragment ions and neutral loss obtained by MS/MS to characterize the chemical composition of Sophora flavescens.