Objective: To determine the genotype of an individual suspected for Aw through DNA sequencing.
Methods: Serologic testing was carried out with standard methods. Exons 6 and 7 of the ABO genes were amplified by PCR and subjected to direct sequencing or sequenced after gene cloning.
Results: Serological testing showed that the forward typing and reverse typing were Aw and A, respectively. DNA sequencing revealed that the individual has carried an Aw allele and an O allele. Haplotype sequencing of each allele has revealed a nt543 variant (543G>C) in the Aw allele.
Conclusion: The individual was verified as a rare A subtype, which was previously unreported in mainland China.