A bifunctional probe reveals increased viscosity and hydrogen sulfide in zebra fish model of Parkinson's disease

Wei Shen; Peng Wang; Zhenda Xie; Haihua Zhou; Yi Hu; Manlin Fu; Qing Zhu

doi:10.1016/j.talanta.2021.122621

A bifunctional probe reveals increased viscosity and hydrogen sulfide in zebra fish model of Parkinson's disease

Talanta. 2021 Nov 1:234:122621. doi: 10.1016/j.talanta.2021.122621. Epub 2021 Jun 26.

Authors

Wei Shen¹, Peng Wang², Zhenda Xie³, Haihua Zhou¹, Yi Hu⁴, Manlin Fu⁵, Qing Zhu⁶

Affiliations

¹ Department of Surgery, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, China.
² Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
³ Institute of Natural Medicine and Health Product, School of Life Science, Taizhou University, Taizhou, 318000, China.
⁴ CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics and University of Chinese Academy of Sciences (UCAS), Chinese Academy of Sciences (CAS), Beijing, 100049, China. Electronic address: huyi@ihep.ac.cn.
⁵ Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China. Electronic address: fublue@163.com.
⁶ Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China. Electronic address: zhuq@zjut.edu.cn.

PMID: 34364430
DOI: 10.1016/j.talanta.2021.122621

Abstract

Altered H₂S levels and intracellular viscosity have both been seen in Parkinson's disease (PD). However, how H₂S and intracellular viscosity are involved in PD pathogenesis remains unknown. Herein, a dual-function fluorescent probe DF was designed and synthesized to analyze intracellular viscosity and hydrogen sulfide. It is a near-infrared fluorescence probe with improved photostability and large Stokes shift (110 nm). The probe reveals increased viscosity and hydrogen sulfide in zebrafish model of PD for the first time.

Keywords: Fluorescent probe; Hydrogen sulfide; Inflammation; Parkinson's disease; Viscosity.

MeSH terms

Animals
Fluorescent Dyes
HeLa Cells
Humans
Hydrogen Sulfide*
Parkinson Disease*
Viscosity
Zebrafish

Substances

Fluorescent Dyes
Hydrogen Sulfide