Use of an optical sensor to directly monitor the metabolic activity of growing bacteria

Abstract

The metabolic activity of growing bacteria was directly monitored by using an electro-optical (EO) sensor. The sensor enables examination of bacteria in batch and continuous cultures. As examples, we report studies with Еscherichia coli, a bacterium with an aerobic type of metabolism, and Lactobacillus plantarum, a bacterium with an anaerobic type of metabolism. Bacterial growth was accompanied by a simultaneous change in both the hydrodynamic mean size (HMS) of the bacteria and the concentration of ions in the cytoplasm (CIC). Both variables were associated with the regulation of cellular metabolic activity, which can be cyclic during intense bacterial growth. A simultaneous change in metabolic activity and osmotic regulation was also found. For СIC and HMS measurements, we used online results of the EO analysis of cells suspended in water. The measured results for the CIC and HMS can be used to directly monitor bacterial metabolism. The results of this study are of practical importance for the real-time EO monitoring of the metabolic activity of growing bacteria without preliminary sample preparation.

Keywords: Bacterial cells; Cytoplasm; Electrical conductivity; Electro-optical sensor; Metabolic activity; Monitoring; Osmotic regulation.