Feeding Systems and Host Breeds Influence Ruminal Fermentation, Methane Production, Microbial Diversity and Metagenomic Gene Abundance

Rajaraman Bharanidharan; Chang Hyun Lee; Krishnaraj Thirugnanasambantham; Ridha Ibidhi; Yang Won Woo; Hong-Gu Lee; Jong Geun Kim; Kyoung Hoon Kim

doi:10.3389/fmicb.2021.701081

Feeding Systems and Host Breeds Influence Ruminal Fermentation, Methane Production, Microbial Diversity and Metagenomic Gene Abundance

Front Microbiol. 2021 Jul 20:12:701081. doi: 10.3389/fmicb.2021.701081. eCollection 2021.

Authors

Rajaraman Bharanidharan¹, Chang Hyun Lee², Krishnaraj Thirugnanasambantham^{3

4}, Ridha Ibidhi³, Yang Won Woo⁵, Hong-Gu Lee⁶, Jong Geun Kim^{3

7}, Kyoung Hoon Kim^{3

7}

Affiliations

¹ Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, South Korea.
² Cargill Agri Purina Inc., Technology Application Center, Pyeongchang, South Korea.
³ Department of Ecofriendly Livestock Science, Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang, South Korea.
⁴ Pondicherry Centre for Biological Science and Educational Trust, Tamil Nadu, India.
⁵ GN Food Ltd., R&D Team, Seoul, South Korea.
⁶ Department of Animal Science and Technology, SangHa Life Science College, Konkuk University, Seoul, South Korea.
⁷ Department of International Agricultural Technology, Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang, South Korea.

Abstract

Our previous research revealed the advantages of separate feeding (SF) systems compared to total mixed ration (TMR) in terms of ruminal methane (CH₄) production. The purpose of this experiment was to confirm the advantage of SF as a nutritional strategy for CH₄ mitigation, and to determine the effects of different feeding systems (TMR and SF) on the rumen microbiome and associated metagenome of two different breeds and on CH₄ emissions. We randomly allocated four Holstein (305 ± 29 kg) and four Hanwoo steers (292 ± 24 kg) to two groups; the steers were fed a commercial concentrate with tall fescue (75:25) as TMR or SF, in a crossover design (two successive 22-day periods). Neither feeding systems nor cattle breeds had an effect on the total tract digestibility of nutrients. The TMR feeding system and Hanwoo steers generated significantly more CH₄ (P < 0.05) and had a higher yield [g/d and g/kg dry matter intake (DMI)] compared to the SF system and Holstein steers. A larger rumen acetate:propionate ratio was observed for the TMR than the SF diet (P < 0.05), and for Hanwoo than Holstein steers (P < 0.001), clearly reflecting a shift in the ruminal H₂ sink toward CH₄ production. The linear discriminant analysis (LDA) effect size (LEfSe) revealed a greater abundance (α < 0.05 and LDA > 2.0) of operational taxonomic units (OTUs) related to methanogenesis for Hanwoo steers compared to Holstein steers. Kendall's correlation analysis revealed wide variation of microbial co-occurrence patterns between feeding systems, indicating differential H₂ thermodynamics in the rumen. A metagenome analysis of rumen microbes revealed the presence of 430 differentially expressed genes, among which 17 and 27 genes exhibited positive and negative associations with CH₄ production, respectively (P < 0.001). A strong interaction between feeding system and breed was observed for microbial and metagenomic abundance. Overall, these results suggest that the TMR feeding system produces more CH₄, and that Hanwoo cattle are higher CH₄ emitters than SF diet and Holstein cattle, respectively. Interestingly, host-associated microbial interactions differed within each breed depending on the feeding system, which indicated that breed-specific feeding systems should be taken into account for farm management.

Keywords: Hanwoo; TMR; metagenome; methane; microbial network; rumen.