Forensic characteristics and population construction of two major minorities from southwest China revealed by a novel 37 Y-STR loci system

Li Luo; Lilan Yao; Siyu Chai; Hao Zhang; Min Li; Jian Yu; Xijie Hu; Chengtao Li; Yingnan Bian; Pengyu Chen

doi:10.1098/rsos.210447

Forensic characteristics and population construction of two major minorities from southwest China revealed by a novel 37 Y-STR loci system

R Soc Open Sci. 2021 Jul 28;8(7):210447. doi: 10.1098/rsos.210447. eCollection 2021 Jul.

Authors

Li Luo^{1

2

3}, Lilan Yao^{1

2}, Siyu Chai^{1

2}, Hao Zhang^{1

2}, Min Li^{3

4}, Jian Yu², Xijie Hu^{1

2}, Chengtao Li^{3

4}, Yingnan Bian³, Pengyu Chen^{1

2}

Affiliations

¹ Key Laboratory of Cell Engineering in Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi 563099, Guizhou, People's Republic of China.
² School of Forensic Medicine, Zunyi Medical University, Zunyi 563099, Guizhou, People's Republic of China.
³ Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, People's Republic of China.
⁴ Institute of Forensic Medicine, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610000, Sichuan, People's Republic of China.

Abstract

Y-chromosome short tandem repeats (Y-STRs) have become important supplementary evidence in forensic science. Nowadays, the Y-chromosome STR haplotype reference database (YHRD) contains abundant Y-STR haplotype data from all over the world, while haplotype data of Guizhou Miao and Tujia are scarce. Hence, genetic polymorphisms of 37 Y-STRs were investigated in 446 unrelated males (206 Miao males and 246 Tujia males) residing in Guizhou Province. A total of 206 and 242 unique haplotypes with the highest diversity value of 0.9665 and 0.9470 were obtained. The heatmap, multidimensional scaling (MDS), the unweighted pair-group method with arithmetic means (UPGMA) tree and principal component analysis (PCA) based on the genetic distance (Rst) value within our studied populations and other 26 populations indicated that population structures follow the boundary of the continent. Guizhou Miao and Guizhou Tujia populations have intimate relationships with East Asian populations, especially the geographically close, similar history and the same language family populations.

Keywords: AGCU Y37 PCR amplification kit; Guizhou Miao; Guizhou Tujia; haplotype; population structure.

Associated data

figshare/10.6084/m9.figshare.c.5525179