The complete chloroplast genome of Ocimum tenuiflorum L. subtype Krishna Tulsi and its phylogenetic analysis

N M Kavya; Raju Balaji; Tanuja; Madasamy Parani; Palanisamy Senthilkumar

doi:10.1080/23802359.2021.1951133

The complete chloroplast genome of Ocimum tenuiflorum L. subtype Krishna Tulsi and its phylogenetic analysis

Mitochondrial DNA B Resour. 2021 Jul 15;6(8):2358-2360. doi: 10.1080/23802359.2021.1951133. eCollection 2021.

Authors

N M Kavya¹, Raju Balaji¹, Tanuja¹, Madasamy Parani¹, Palanisamy Senthilkumar¹

Affiliation

¹ Center for DNA Barcoding, Department of Genetic Engineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

Abstract

The complete chloroplast genome (cp) of Ocimum tenuiflorum L. subtype Krishna Tulsi was sequenced and assembled using Illumina paired-end sequencing data. The cp genome is 151,758 bp in length, including a large single copy (LSC) region of 82,794 bp, a small single-copy region (SSC) of 17,592 bp, and a pair of inverted repeated (IR) region of 25,686 bp. The cp genome of Krishna Tulsi encodes 129 genes, including 90 protein-coding, 31 transfer RNA (tRNA), and eight ribosomal RNA (rRNA) genes. While the overall GC content was 37.9%, it is 36.0%, 31.8%, and 43.1% in the LSC, SSC, and IR regions, respectively. Phylogenetic analysis based on chloroplast genome sequences of 17 species from Lamiaceae revealed that O. tenuiflorum subtype Krishna Tulsi is clustered with other Ocimum species, and forms a clade with genera from family Lamiaceae.

Keywords: Complete chloroplast genome; Krishna Tulsi; Lamiaceae; Ocimum tenuiflorum; phylogenetic analysis.

Grants and funding

We would like to acknowledge the funding from the SRM-DBT Partnership Platform for Contemporary Research Services and Skill Development in Advanced Life Sciences Technologies [Grant No. BT/PR12987/INF/22/205/2015].