Complete mitochondrial genome of Coscinodiscus granii (Coscinodiscophyceae, Bacillariophyta)

Hailong Huang; Huiyin Song; Zengxia Zhao; Feng Liu; Nansheng Chen

doi:10.1080/23802359.2021.1951135

Complete mitochondrial genome of Coscinodiscus granii (Coscinodiscophyceae, Bacillariophyta)

Mitochondrial DNA B Resour. 2021 Jul 15;6(8):2332-2334. doi: 10.1080/23802359.2021.1951135. eCollection 2021.

Authors

Hailong Huang^{1

2

3}, Huiyin Song^{1

2

3}, Zengxia Zhao^{2

4}, Feng Liu^{1

2

3}, Nansheng Chen^{1

2

5

3}

Affiliations

¹ CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
² Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
³ Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, Canada.
⁴ Jiaozhou Bay National Marine Ecosystem Research Station, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
⁵ Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China.

Abstract

Coscinodiscus is a genus common in marine phytoplankton, with some species thought to have a significant negative ecological impact. However, the availability of their genome sequences is rather limited. Here, we assembled and annotated the first complete mitochondrial genome (mtDNA) of the species Coscinodiscus granii L.F.Gough 1905, as part of our efforts to gain a better understanding of the genetic characteristics of Coscinodiscus taxa at a genomic level. The circular mtDNA was 34,970 bp in length and encoded 60 genes, including 32 protein-coding genes (PCGs), 24 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and two conserved open reading frames (orfs). The overall GC content of C. granii mtDNA was 24.30%, which was slightly lower than that of C. wailesii (25.00%), the first species in the genus Coscinodiscus whose mtDNA has been reported, and higher than that of Melosira undulata (21.60%), the first species in the class Coscinodiscophyceae whose mtDNA has been reported. As expected for congeneric species, phylogenetic analysis using concatenated amino acid sequences of 27 shared PCGs suggested that C. granii has a closer evolutionary relationship with C. wailesii. Coscinodiscus was found to be monophyletic in the phylogeny. The complete mtDNAs of more Coscinodiscus species will facilitate the exploration of the evolutionary relationships of species in the Class Coscinodiscophyceae.

Keywords: Coscinodiscophyceae; Coscinodiscus granii; Diatoms; mitochondrial genome.

Grants and funding

This research was supported by the Strategic Priority Research Program of Chinese Academy of Sciences [Grant No. XDB42000000], the Chinese Academy of Sciences Pioneer Hundred Talents Program (to Nansheng Chen), the Taishan Scholar Project Special Fund (to Nansheng Chen), the Qingdao Innovation and Creation Plan (Talent Development Program – 5th Annual Pioneer and Innovator Leadership Award to Nansheng Chen, 19-3-2-16-zhc), the Key Research Program of Frontier Sciences, Chinese Academy of Sciences [No. QYZDB-SSW-DQC023] (to Feng Liu), and the Major Scientific and Technological Innovation Project of Shandong Province [No. 2019JZZY020706] (to Feng Liu), the Post-doctoral Program of Qingdao 2020 [Grant No. E0KY061] (to Hailong Huang), the Natural Science Foundation of Shandong Province, China [Grant No. ZR2020QD095] (to Hailong Huang).