This article aims to stabilize probabilistic Boolean networks (PBNs) via a novel pinning control strategy. In a PBN, the state evolution of each gene switches among a collection of candidate Boolean functions with preassigned probability distributions, which govern the activation frequency of each Boolean function. Due to the existence of stochasticity, the mode-independent pinning controller might be disabled. Thus, both mode-independent and mode-dependent pinning controller are required here. Moreover, a criterion is derived to determine whether mode-independent controllers are applicable while the pinned nodes are given. It is worth pointing out that this pinning control is based on the n×n network structure rather than 2n ×2n state transition matrix. Therefore, compared with the existing results, this pinning control strategy is more practicable and has the ability to handle large-scale networks, especially sparsely connected networks. To demonstrate the effectiveness of the designed control scheme, a PBN that describes the mammalian cell-cycle encountering a mutated phenotype is discussed as a simulation.