Hydrogen sulfide and nitric oxide are involved in melatonin-induced salt tolerance in cucumber

Abstract

Hydrogen sulfide (H₂S) is a novel gaseous signaling molecule in response to adversity stress. Melatonin (MT) is a multifunctional molecule that plays an important role in regulating plant stress resistance. However, the interactions between H₂S and MT are still unknown. Therefore, the role of H₂S in MT-induced salt tolerance was elucidated in this study by measuring the antioxidant defense system and photosynthetic characteristics of cucumber. In addition, the crosstalk among H₂S, NO, and mitogen-activated protein kinase (MAPK) was investigated. Results showed that MT induced the production of H₂S by significantly increasing the activity of L-/D-cysteine desulfhydrase, thereby regulating photosynthetic efficiency, antioxidant enzyme activity, and antioxidant enzyme gene expression in cucumber, thus alleviating reactive oxygen species burst by salt stress. In this process, the H₂S and NO induced by MT were inhibited by NO scavenger (cPTIO) and H₂S scavenger (HT) but not affected by MAPK inhibitor (U0126). Intriguingly, the expression of MAPK3/4/6/9 was inhibited by HT and cPTIO. These results suggested that H₂S may act as downstream of MT, interact with NO and MAPK cascades, and jointly participate in the process of MT mitigating salt stress in cucumber. In addition, H₂S and NO are upstream signaling molecules of the MAPK cascades.

Keywords: Cucumber; Hydrogen sulfide; Melatonin; Salt stress.