Recent advances in FRET-Based biosensors for biomedical applications

Mahsa Imani; Nasrin Mohajeri; Mojgan Rastegar; Nosratollah Zarghami

doi:10.1016/j.ab.2021.114323

Recent advances in FRET-Based biosensors for biomedical applications

Anal Biochem. 2021 Oct 1:630:114323. doi: 10.1016/j.ab.2021.114323. Epub 2021 Jul 31.

Authors

Mahsa Imani¹, Nasrin Mohajeri², Mojgan Rastegar³, Nosratollah Zarghami⁴

Affiliations

¹ Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
² Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Mohajerin@tbzmed.ac.ir.
³ Department of Biochemistry and Medical Genetics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada; Regenerative Medicine Program, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada.
⁴ Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medicine, Faculty of Medicine, Istanbul Aydın University, Istanbul, Turkey; Department of Clinical Biochemistry and Laboratory Medicine, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Zarghamin@gmail.com.

PMID: 34339665
DOI: 10.1016/j.ab.2021.114323

Abstract

Fluorescence resonance energy transfer (FRET)-based biosensors are effective analytical tools extensively used in fields of biomedicine, pharmacology, toxicology, and food sciences. Ratiometric imaging of substantial cellular processes, molecular components, and biological interactions is widely performed by these biosensors. A variety of FRET-based biosensors have provided comprehensive insights into underlying mechanisms of pathological conditions in live cells, tissues, and organisms. Moreover, integration of FRET-based biosensors with the current bioanalytical techniques allows for accurate, rapid, and sensitive diagnosis and proposes the advanced strategies for treatment. Precise analysis of ligand-receptor interactions by FRET-based biosensors has presented a basis for determination of novel therapeutic agents. Therefore, this study was designed to review the recent developments in FRET-based biosensors and their biomedical applications. In addition, characteristics, challenges, and outlooks of these biosensors were discussed.

Keywords: Biomedical application; Biosensor; Cancer biomarker; Cellular imaging; FRET; Ratiometric imaging.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biomedical Research*
Biosensing Techniques*
Fluorescence Resonance Energy Transfer*
Fluorescent Dyes / chemistry*
Humans

Substances

Fluorescent Dyes