Evaluation of AMPK activity in mice by measuring activator-induced glucose uptake

STAR Protoc. 2021 Jul 17;2(3):100496. doi: 10.1016/j.xpro.2021.100496. eCollection 2021 Sep 17.

Abstract

The AMP-activated protein kinase (AMPK) is a principal nutrient sensor and a master regulator of cellular energy homeostasis. Once activated, AMPK induces glucose uptake, which leads to a transient decrease in blood glucose level and can be used as an indicator of AMPK activity. Here, we present a protocol accessing AMPK activity in mice by measuring glucose uptake induced by AMPK activators, MK8722 and A769662. This protocol can be used to evaluate AMPK signaling in vivo under various pathophysiological conditions. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).

Keywords: Metabolism; Model Organisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylate Kinase / metabolism*
  • Animals
  • Benzimidazoles / pharmacology*
  • Biphenyl Compounds / pharmacology*
  • Enzyme Activators / pharmacology*
  • Glucose / metabolism*
  • Male
  • Mice
  • Pyridines / pharmacology*
  • Pyrones / pharmacology*
  • Thiophenes / pharmacology*

Substances

  • Benzimidazoles
  • Biphenyl Compounds
  • Enzyme Activators
  • MK-8722
  • Pyridines
  • Pyrones
  • Thiophenes
  • Adenylate Kinase
  • Glucose
  • 4-hydroxy-3-(4-(2-hydroxyphenyl)phenyl)-6-oxo-7H-thieno(2,3-b)pyridine-5-carbonitrile