Simultaneous Determination of Hydrochlorothiazide and Losartan Potassium in Pharmaceutical Product by UV-Vis Spectrophotometric Method with Kalman Filter Algorithm

Tran Thuc Binh; Le Thi Phuong Tram; Nguyen Van Hop; Nguyen Dang Giang Chau; Nguyen Duy Luu; Nguyen Thi Quynh Trang

doi:10.1155/2021/2754133

Simultaneous Determination of Hydrochlorothiazide and Losartan Potassium in Pharmaceutical Product by UV-Vis Spectrophotometric Method with Kalman Filter Algorithm

J Anal Methods Chem. 2021 Jul 20:2021:2754133. doi: 10.1155/2021/2754133. eCollection 2021.

Authors

Tran Thuc Binh¹, Le Thi Phuong Tram¹, Nguyen Van Hop¹, Nguyen Dang Giang Chau¹, Nguyen Duy Luu², Nguyen Thi Quynh Trang³

Affiliations

¹ University of Sciences, Hue University, Hue 530000, Vietnam.
² Da Nang University of Medical Technology and Pharmacy, Da Nang 550000, Vietnam.
³ Faculty of Environmental Sciences, Saigon University, Ho Chi Minh City 700000, Vietnam.

Abstract

In this paper, hydrochlorothiazide (HCT) and losartan potassium (LSP) in tablets are simultaneously determined with UV-Vis spectrophotometry and chemometrics without separation. The spectra of standard and sample solutions were recorded in the wavelength from 220 to 300 nm at 1.0 nm intervals. The concentrations of HCT and LSP in the sample solutions were computed with the Kalman filter algorithm written on the Microsoft Excel 2016 and Visual Basic for Applications (VBA) platform. The method validation was determined via the accuracy and repeatability of measurements when analyzing HCT and LSP in the Splozarsin Plus tablet and comparing the mean values of their contents in the sample with those analyzed with HPLC. The proposed method is simple with a low cost compared with the standard HPLC method.