Crataegus monogyna is an important plant of the Rosaceae family, widely used in traditional medicine to treat various conditions such as cardiovascular diseases, cancer, diabetes, asthma, and nephritis. The aim of the current study was to assess the chemical composition, antioxidant, and antibacterial activity of leaves' extracts against Bacillus cereus, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The total amounts of polyphenols and flavonoids contained in the dry extracts of plants were estimated by colorimetric methods. DPPH assay was utilized to measure the antioxidant activity of C. monogyna. The phytochemical compounds were determined through HPLC technique, and the minimum inhibitory concentration (MIC) of ethanol extract was performed using the broth dilution method. The ethanol extract represented the richest extract in polyphenol with 473.4 mg GAE g-1 and flavonoids 80.9 mg CE g-1 and showed considerable antioxidant potential IC50 =22.50 µg/ml. The antibacterial susceptibility test against Staphylococcus aureus 0.512 mg/mL.
Keywords: Crataegus monogyna; DPPH assay; HPLC; MIC.