Molecular hybridization combining tumor homing and penetrating peptide domains for cellular targeting

Ruchika Goyal; Gaurav Jerath; Aneesh Chandrasekharan; Yvonne Christian; T R Santhosh Kumar; Vibin Ramakrishnan

doi:10.1007/s13346-021-01035-z

Molecular hybridization combining tumor homing and penetrating peptide domains for cellular targeting

Drug Deliv Transl Res. 2022 May;12(5):1285-1292. doi: 10.1007/s13346-021-01035-z. Epub 2021 Jul 31.

Authors

Ruchika Goyal¹, Gaurav Jerath¹, Aneesh Chandrasekharan², Yvonne Christian¹, T R Santhosh Kumar², Vibin Ramakrishnan³

Affiliations

¹ Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
² Rajiv Gandhi Centre for Biotechnology, Cancer Research Program-1, Thiruvananthapuram, 695014, Kerala, India.
³ Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India. vibin@iitg.ac.in.

PMID: 34333729
DOI: 10.1007/s13346-021-01035-z

Abstract

A complete peptide-based drug delivery unit has been designed with a tumor homing domain chemically linked to a syndiotactic cell-penetrating domain. The designed peptides were synthesized, characterized, and tested in vitro for cellular uptake and cytotoxicity evaluation. The differential uptake, cellular internalization, negligible hemotoxicity, selective toxicity to MDA-MB-231 breast cancer cells, and the superior penetration in three-dimensional MDA-MB-231 tumorospheres confirm their utility as a promising delivery vector.

Keywords: Cell-penetrating peptide; Drug delivery; Drug design; Tumor homing peptide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents* / chemistry
Cell Line, Tumor
Cell-Penetrating Peptides* / chemistry
Drug Delivery Systems
Protein Domains

Substances

Antineoplastic Agents
Cell-Penetrating Peptides