Hydroxylamine metabolism in mainstream denitrifying ammonium oxidation (DEAMOX) process: Achieving fast start-up and robust operation with bio-augmentation assistance under ambient temperature

Xingxing Zhang; Xiaonong Zhang; Peng Wu; Liping Ma; Junjiang Chen; Chaochao Wang; Xiang Li; Wenru Liu; Lezhong Xu

doi:10.1016/j.jhazmat.2021.126736

Hydroxylamine metabolism in mainstream denitrifying ammonium oxidation (DEAMOX) process: Achieving fast start-up and robust operation with bio-augmentation assistance under ambient temperature

J Hazard Mater. 2022 Jan 5:421:126736. doi: 10.1016/j.jhazmat.2021.126736. Epub 2021 Jul 24.

Authors

Xingxing Zhang¹, Xiaonong Zhang², Peng Wu³, Liping Ma⁴, Junjiang Chen², Chaochao Wang², Xiang Li⁵, Wenru Liu⁵, Lezhong Xu⁵

Affiliations

¹ School of Environmental Science and Engineering, Suzhou University of Science and Technology, No. 1 Kerui Road, Suzhou 215009, China; Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China.
² School of Environmental Science and Engineering, Suzhou University of Science and Technology, No. 1 Kerui Road, Suzhou 215009, China.
³ School of Environmental Science and Engineering, Suzhou University of Science and Technology, No. 1 Kerui Road, Suzhou 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, No. 1 Kerui Road, Suzhou 215009, China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, No. 1 Kerui Road, Suzhou 215009, China. Electronic address: wupengniu@126.com.
⁴ Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China. Electronic address: lpma@des.ecnu.edu.cn.
⁵ School of Environmental Science and Engineering, Suzhou University of Science and Technology, No. 1 Kerui Road, Suzhou 215009, China; National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, No. 1 Kerui Road, Suzhou 215009, China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, No. 1 Kerui Road, Suzhou 215009, China.

PMID: 34333411
DOI: 10.1016/j.jhazmat.2021.126736

Abstract

Nitrogen removal from mainstream wastewater via DEnitrifying AMmonium OXidation (DEAMOX) is often challenged by undulated actual temperature and high loading rate. Here, we discovered NH₂OH addition (HA) and bio-augmentation (BA) tactics on start-up and operation performance of DEAMOXs (R1 and R2) under ambient temperature (11.3-31.7 °C). Over 340-day operation suggested that R2 received 10 mg/L HA and 1:25 BA ratio (v/v, anammox/partial denitrification sludge) achieved desirable nitrogen removal efficiency (NRE) of 97.22% after 145-day, while R1 under higher BA ratio of 1:12.5 without HA obtained lower NRE (90.86%) after 184-day. Batch tests revealed that nitrate-nitrite transformation ratio reached 98.64% at low COD/NO₃^--N of 2.6 with HA. Significantly, compared with R2, R1 recovered quickly with satisfactory effluent total nitrogen of 4.21 mg/L despite nitrogen loading rate greater than 0.15 kg N/m³/d and temperature decreased to 14.6 °C. The abundant narG represented high nitrate reduction potential, hzsA and hdh were extensively detected as the symbolisation of anammox metabolism. Thauera, Denitratisoma and unclassified f Comamonadaceae dominated nitrite accumulation. Ca. Brocadia as the dominant anammox bacteria, and its population maintained stable against low temperature and load shocks by NH₂OH intensification. Overall, this study offers an opportunity for the wide-applications of DEAMOX treating mainstream wastewater.

Keywords: Bioaugmented DEAMOX; Biological nitrogen removal; Fast start-up; Hydroxylamine (NH(2)OH) metabolism; Nitrite accumulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ammonium Compounds*
Bioreactors
Denitrification*
Hydroxylamine
Hydroxylamines
Nitrogen / analysis
Oxidation-Reduction
Sewage
Temperature
Wastewater

Substances

Ammonium Compounds
Hydroxylamines
Sewage
Waste Water
Hydroxylamine
Nitrogen