Multimodal Approach for Cancer Cell Investigation

Alexandre Berquand; Jerôme Devy

doi:10.1007/978-1-0716-1593-5_18

Multimodal Approach for Cancer Cell Investigation

Methods Mol Biol. 2021:2350:289-297. doi: 10.1007/978-1-0716-1593-5_18.

Authors

Alexandre Berquand¹, Jerôme Devy²

Affiliations

¹ Laboratoire de Recherche en Nanosciences LRN EA4682 and NanoMat' platform, Université de Reims Champagne-Ardenne, Reims, France. alexandre.berquand@univ-reims.fr.
² CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire (MEDyC), UFR Sciences Exactes et Naturelles, Université de Reims Champagne-Ardenne (URCA), Laboratoire SiRMa - Campus Moulin de la Housse, Reims cedex, France.

PMID: 34331292
DOI: 10.1007/978-1-0716-1593-5_18

Abstract

Atomic force microscopy (AFM) enables the characterization of a wide range of samples including live cells. It is generally admitted that cancer cells are significantly softer than their normal counterparts, but imaging live cells by AFM using traditional modes can be at the cost of time or resolution. We describe how this tool can be used to estimate the motility of cancer versus normal cells, based on topographical and mechanical approaches, and coupled to optical imaging.

Keywords: Atomic force microscopy; Cancer; Fluorescence imaging; Force measurements; PeakForce QNM; Video microscopy.

MeSH terms

Cell Line, Tumor
Cell Movement*
Cells, Cultured
Fluorescent Antibody Technique / methods
Humans
Microscopy, Atomic Force* / methods
Microscopy, Video / methods*
Neoplasms / pathology*
Optical Imaging / methods*