RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells

Hongjiang Si; Xu Peng

doi:10.1007/978-1-0716-1480-8_3

RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells

Methods Mol Biol. 2021:2319:25-30. doi: 10.1007/978-1-0716-1480-8_3.

Authors

Hongjiang Si¹, Xu Peng^{2

3}

Affiliations

¹ Department of Medical Physiology, College of Medicine, Texas A&M University, Bryan, TX, USA.
² Department of Medical Physiology, College of Medicine, Texas A&M University, Bryan, TX, USA. xp23@tamu.edu.
³ Department of Medical Physiology, College of Medicine, Texas A&M University of Health Science Center, Bryan, TX, USA. xp23@tamu.edu.

PMID: 34331239
DOI: 10.1007/978-1-0716-1480-8_3

Abstract

Fluid shear stress (FSS) is an important mechanical force that regulates endothelial and vascular functions in the blood and lymphatic vasculature. RNA-sequencing (RNA-Seq), an emerging next-generation sequencing (NGS) technique, facilitates us to quantify the global gene expression levels and, thereby, provides us with a powerful tool to study the transcriptional reprograming in the cells. Here, we describe the RNA-Seq procedures and the subsequent bioinformatic analysis to understand the transcriptome regulation by FSS in lymphatic endothelial cells (LECs).

Keywords: Fluid shear stress; Lymphatic endothelial cells; RNA-sequencing; Transcriptome.

MeSH terms

Cells, Cultured
Endothelial Cells / metabolism*
Gene Expression Profiling / methods*
Humans
RNA-Seq
Stress, Mechanical
Stress, Physiological / genetics*
Transcriptome / genetics*