Differential response of immobile (pneumocytes) and mobile (monocytes) barriers against 2 types of metal oxide nanoparticles

Abstract

Background: Inhaled nanoparticles (NPs) challenges mobile and immobile barriers in the respiratory tract, which can be represented by type II pneumocytes (immobile) and monocytes (mobile) but what is more important for biological effects, the cell linage, or the type of nanoparticle? Here, we addressed these questions and we demonstrated that the type of NPs exerts a higher influence on biological effects, but cell linages also respond differently against similar type of NPs.

Design: Type II pneumocytes and monocytes were exposed to tin dioxide (SnO₂) NPs and titanium dioxide (TiO₂) NPs (1, 10 and 50 μg/cm²) for 24 h and cell viability, ultrastructure, cell granularity, molecular spectra of lipids, proteins and nucleic acids and cytoskeleton architecture were evaluated.

Results: SnO₂ NPs and TiO₂ NPs are metal oxides with similar physicochemical properties. However, in the absence of cytotoxicity, SnO₂ NPs uptake was low in monocytes and higher in type II pneumocytes, while TiO₂ NPs were highly internalized by both types of cells. Monocytes exposed to both types of NPs displayed higher number of alterations in the molecular patterns of proteins and nuclei acids analyzed by Fourier-transform infrared spectroscopy (FTIR) than type II pneumocytes. In addition, cells exposed to TiO₂ NPs showed more displacements in FTIR spectra of biomolecules than cells exposed to SnO₂ NPs. Regarding cell architecture, microtubules were stable in type II pneumocytes exposed to both types of NPs but actin filaments displayed a higher number of alterations in type II pneumocytes and monocytes exposed to SnO₂ NPs and TiO₂ NPs. NPs exposure induced the formation of large vacuoles only in monocytes, which were not seen in type II pneumocytes.

Conclusions: Most of the cellular effects are influenced by the NPs exposure rather than by the cell type. However, mobile, and immobile barriers in the respiratory tract displayed differential response against SnO₂ NPs and TiO₂ NPs in absence of cytotoxicity, in which monocytes were more susceptible than type II pneumocytes to NPs exposure.

Keywords: Cytoskeleton; FTIR spectra; Monocytes; Tin dioxide; Titanium dioxide; Type II pneumocytes.