CRISPR: History and perspectives to the future

Z Kozovska; S Rajcaniova; P Munteanu; S Dzacovska; L Demkova

doi:10.1016/j.biopha.2021.111917

CRISPR: History and perspectives to the future

Biomed Pharmacother. 2021 Sep:141:111917. doi: 10.1016/j.biopha.2021.111917. Epub 2021 Jul 16.

Authors

Z Kozovska¹, S Rajcaniova², P Munteanu³, S Dzacovska⁴, L Demkova⁵

Affiliations

¹ Department of Molecular Oncology, Cancer Research Institute, Biomedical Research Center, University Science Park for Biomedicine, Slovak Academy of Sciences, Dubravska cesta 9, 84505 Bratislava, Slovakia. Electronic address: zuzana.kozovska@savba.sk.
² Department of Cell and Molecular Biology of Drugs Faculty of Pharmacy, Comenius University, Odbojarov 10, 83232 Bratislava, Slovakia.
³ Institute of Biochemistry and Microbiology, Faculty of chemical and food technology, Slovak Technical University, Radlinského 9, 81237 Bratislava, Slovakia.
⁴ Department of Genetics, Faculty of Natural Sciences, Comenius University, Ilkovicova 6, 84215 Bratislava, Slovakia.
⁵ Department of Molecular Oncology, Cancer Research Institute, Biomedical Research Center, University Science Park for Biomedicine, Slovak Academy of Sciences, Dubravska cesta 9, 84505 Bratislava, Slovakia.

PMID: 34328110
DOI: 10.1016/j.biopha.2021.111917

Abstract

This review summarizes the information about the history and future of the CRISPR/Cas9 method. Genome editing can be perceived as a group of technologies that allow scientists to change the DNA of an organism. These technologies involve the deletion, insertion, or modification of the genome at a specific site in a DNA sequence. Gene therapy in humans has a perspective to be used to eliminate the gene responsible for a particular genetic disorder. The review focuses on the key elements of this promising method and the possibility of its application in the treatment of cancer and genetic diseases.

Keywords: CRISPR; Cas endonucleases; Gene engineering; On or off-targets effects.

Publication types

Review

MeSH terms

Animals
CRISPR-Associated Protein 9 / genetics*
CRISPR-Associated Protein 9 / metabolism
CRISPR-Cas Systems / physiology*
Forecasting
Gene Editing / methods*
Gene Editing / trends
Genetic Engineering / methods
Genetic Engineering / trends
Genetic Therapy / methods*
Genetic Therapy / trends
Humans

Substances

CRISPR-Associated Protein 9