A draft reference assembly of the Psilocybe cubensis genome

F1000Res. 2021 Apr 9:10:281. doi: 10.12688/f1000research.51613.2. eCollection 2021.

Abstract

We describe the use of high-fidelity single molecule sequencing to assemble the genome of the psychoactive Psilocybe cubensis mushroom. The genome is 46.6Mb, 46% GC, and in 32 contigs with an N50 of 3.3Mb. The BUSCO completeness scores are 97.6% with 1.2% duplicates. The Psilocybin synthesis cluster exists in a single 3.2Mb contig. The dataset is available from NCBI BioProject with accessions PRJNA687911 and PRJNA700437.

Keywords: Genome; Psilocybe cubensis; Psilocybin; Single molecule sequencing.

MeSH terms

  • Agaricales* / genetics
  • Psilocybe*
  • Psilocybin

Substances

  • Psilocybin

Supplementary concepts

  • Psilocybe cubensis