Abstract
We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible Lox site pairs. Following Cre-mediated recombination, the genotypes of single and double transgenic individuals were successfully identified by specific marker combinations in 461 scorings.
© 2021. The Author(s).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified*
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Embryo, Nonmammalian
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Female
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism
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Gene Expression
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Genetic Engineering / economics
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Genetic Engineering / methods*
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Genetic Markers
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Genetic Vectors / chemistry
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Genetic Vectors / metabolism*
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Genotyping Techniques*
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Heterozygote
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Histones / genetics
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Histones / metabolism
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Homozygote
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Integrases / genetics*
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Integrases / metabolism
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Male
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Microscopy, Fluorescence
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Promoter Regions, Genetic
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Tribolium / genetics*
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Tubulin / genetics
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Tubulin / metabolism
Substances
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Fluorescent Dyes
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Genetic Markers
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Histones
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Tubulin
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Cre recombinase
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Integrases