Nanoparticle-associated single step hydrogen fermentation for the conversion of starch potato waste biomass by thermophilic Parageobacillus thermoglucosidasius

Abstract

In the present study, starch-based potato peel waste biomass (PWB) was utilized as a potential substrate for hydrogen production via dark fermentation by the thermophillic amylase producing strain Parageobacillus thermoglucosidasius KCTC 33548. Supplementation of Fe₃O₄ nanoparticles (300 mg/L) led to a 4.15-fold increase in hydrogen production as compared to the control. The addition of optimized concentrations of both Fe₃O₄ nanoparticles (300 mg/L) and L-cysteine (250 mg/L) during hydrogen fermentation using pure starch and PWB generated maximum cumulative hydrogen yields of 167 and 71.9 mL with maximum production rates of 2.81 and 1.26 mL/h, respectively. Further, the correlation between Fe₃O₄ and the expression of hydrogenase isoforms and the related hydrogenase activity was explored. The possible mechanisms of the action of Fe₃O₄ on enhanced hydrogenase activity and hydrogen production was elucidated. To our knowledge, there are no such studies reported on enhanced hydrogen production from PWB in a single step.

Keywords: Dark fermentation; Fe(3)O(4) nanoparticles; Hydrogen; Hydrogenase; Starch, Potato peel waste.