Overexpressing CCW12 in Saccharomyces cerevisiae enables highly efficient ethanol production from lignocellulose hydrolysates

Meilin Kong; Xiaowei Li; Tongtong Li; Xuebing Zhao; Mingjie Jin; Xin Zhou; Hanqi Gu; Vladimir Mrša; Wei Xiao; Limin Cao

doi:10.1016/j.biortech.2021.125487

Overexpressing CCW12 in Saccharomyces cerevisiae enables highly efficient ethanol production from lignocellulose hydrolysates

Bioresour Technol. 2021 Oct:337:125487. doi: 10.1016/j.biortech.2021.125487. Epub 2021 Jul 2.

Authors

Meilin Kong¹, Xiaowei Li¹, Tongtong Li¹, Xuebing Zhao², Mingjie Jin³, Xin Zhou⁴, Hanqi Gu⁵, Vladimir Mrša⁶, Wei Xiao⁷, Limin Cao⁸

Affiliations

¹ Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China.
² Key Laboratory for Industrial Biocatalysis, Ministry of Education of China, Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
³ School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
⁴ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
⁵ Department of Biology and Food Science, Hebei Normal University for Nationalities, Chengde, Hebei 067000, China.
⁶ Laboratory of Biochemistry, Faculty of Food Technology and Biotechnology, University of Zagreb, 10000 Zagreb, Croatia.
⁷ Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China; Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK S7N 5E5, Canada.
⁸ Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China. Electronic address: caolimin@cnu.edu.cn.

PMID: 34320766
DOI: 10.1016/j.biortech.2021.125487

Abstract

A Saccharomyces cerevisiae strain CCW12^OE was constructed by overexpressing CCW12 in a previously reported strain WXY70 harboring six xylose utilization genes. CCW12^OE produced an optimal ethanol yield of 98.8% theoretical value within 48 h in a simulated corn stover hydrolysate. CCW12^OEwas comprehensively evaluated for ethanol production in Miscanthus, maize and corncob hydrolysates, among which a 96.1% theoretical value was achieved within 12 h in corncob hydrolysates. Under normal growth conditions, CCW12^OE did not display altered cell morphology; however, in the presence of acetate, CCW12^OE maintained relatively intact cell structure and increased cell wall thickness by nearly 50%, while WXY70 had abnormal cell morphology and reduced cell wall thickness by nearly 50%. Besides, CCW12^OE had higher fermentation capacity than that of WXY70 in undetoxified and detoxified hydrolysates with both aerobic and anaerobic conditions, demonstrating that CCW12 overexpression alone exhibits improved stress resistance and better fermentation performance.

Keywords: CCW12; Ethanol; Lignocellulosic; Saccharomyces cerevisiae; Xylose.

MeSH terms

Ethanol*
Fermentation
Lignin / metabolism
Saccharomyces cerevisiae* / genetics
Saccharomyces cerevisiae* / metabolism
Xylose

Substances

lignocellulose
Ethanol
Lignin
Xylose