The upstream 5' splice site remains associated to the transcription machinery during intron synthesis

Nat Commun. 2021 Jul 27;12(1):4545. doi: 10.1038/s41467-021-24774-6.

Abstract

In the earliest step of spliceosome assembly, the two splice sites flanking an intron are brought into proximity by U1 snRNP and U2AF along with other proteins. The mechanism that facilitates this intron looping is poorly understood. Using a CRISPR interference-based approach to halt RNA polymerase II transcription in the middle of introns in human cells, we discovered that the nascent 5' splice site base pairs with a U1 snRNA that is tethered to RNA polymerase II during intron synthesis. This association functionally corresponds with splicing outcome, involves bona fide 5' splice sites and cryptic intronic sites, and occurs transcriptome-wide. Overall, our findings reveal that the upstream 5' splice sites remain attached to the transcriptional machinery during intron synthesis and are thus brought into proximity of the 3' splice sites; potentially mediating the rapid splicing of long introns.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pairing / genetics
  • Base Sequence
  • Exons / genetics
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Introns / genetics*
  • Microfilament Proteins / genetics
  • RNA Polymerase II / metabolism
  • RNA Precursors / genetics
  • RNA Precursors / metabolism
  • RNA Splice Sites / genetics*
  • RNA, Small Nuclear / genetics
  • RNA, Small Nuclear / metabolism
  • RNA-Binding Proteins / genetics
  • Ribonucleoprotein, U1 Small Nuclear / metabolism
  • Transcription, Genetic*
  • Transcriptome / genetics

Substances

  • FRG1 protein, human
  • Microfilament Proteins
  • RNA Precursors
  • RNA Splice Sites
  • RNA, Small Nuclear
  • RNA-Binding Proteins
  • Ribonucleoprotein, U1 Small Nuclear
  • U1 small nuclear RNA
  • RNA Polymerase II